甲狀腺球蛋白(TG)重組蛋白
Organism species | Mus musculus (Mouse) |
Product No. | KL355Mu01 |
Source | Prokaryotic expression |
Host | E.coli |
Purity | > 95% |
UOM | 50ug |
Predicted Molecular Mass | 30.6kDa |
Concentration | 7.8 |
Applications | SDS-PAGE; WB; ELISA; IP. |
Endotoxin Level | <1.0EU per 1μg (determined by the LAL method) |
Residues | Ser2418~Asn2660 (Accession # O08710) with two N-terminal Tags, His-tag and T7-tag |
Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SPA AIISPERAQQ QAAALAKEVG CPTSSIQEVV SCLRQKPANI LNDAQTKLLA VSGPFHYWGP VVDGQYLREL PSRRLKRPLP VKVDLLIGGS QDDGLINRAK AVKQFEESQG RTNSKTAFYQ ALQNSLGGED SDARILAAAV WYYSLEHSTD DYASFSRALE NATRDYFIIC PMVNMASLWA RRTRGNVFMY HVPESYGHGS LELLADVQYA FGLPFYSAYQ GQFSTEEQSL SLKVMQYFSN
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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