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詳情介紹：
拓?fù)洚悩?gòu)酶Ⅱ(TOP2)重組蛋白
Recombinant Topoisomerase II (TOP2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | KL792Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 30.3kDa |
| Concentration | 6.2 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1μg (determined by the LAL method) |
| Residues | Ile923~Arg1148 (Accession # P11388) with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
拓?fù)洚悩?gòu)酶Ⅱ(TOP2)重組蛋白SEQUENCES
The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ISELPVRT WTQTYKEQVL EPMLNGTEKT PPLITDYREY HTDTTVKFVV KMTEEKLAEA ERVGLHKVFK LQTSLTCNSM VLFDHVGCLK KYDTVLDILR DFFELRLKYY GLRKEWLLGM LGAESAKLNN QARFILEKID GKIIIENKPK KELIKVLIQR GYDSDPVKAW KEAQQKVPDE EENEESDNEK ETEKSDSVTD SGPTFNYLLD MPLWYLTKEK KDELCRLR
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ISELPVRT WTQTYKEQVL EPMLNGTEKT PPLITDYREY HTDTTVKFVV KMTEEKLAEA ERVGLHKVFK LQTSLTCNSM VLFDHVGCLK KYDTVLDILR DFFELRLKYY GLRKEWLLGM LGAESAKLNN QARFILEKID GKIIIENKPK KELIKVLIQR GYDSDPVKAW KEAQQKVPDE EENEESDNEK ETEKSDSVTD SGPTFNYLLD MPLWYLTKEK KDELCRLR
拓?fù)洚悩?gòu)酶Ⅱ(TOP2)重組蛋白USAGE
Reconstitute in sterile ddH2O.
拓?fù)洚悩?gòu)酶Ⅱ(TOP2)重組蛋白STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
拓?fù)洚悩?gòu)酶Ⅱ(TOP2)重組蛋白About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
KL025Ra01 角蛋白8(KRT8)重組蛋白 Recombinant Keratin 8 (KRT8) Rattus norvegicus (Rat)Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
KL025Ra02 角蛋白8(KRT8)重組蛋白 Recombinant Keratin 8 (KRT8) Rattus norvegicus (Rat)
KL036Ra02 轉(zhuǎn)鐵蛋白(TRF)重組蛋白 Recombinant Transferrin (TRF) Rattus norvegicus (Rat)
KL340Bo02 胱天蛋白酶6(CASP6)重組蛋白 Recombinant Caspase 6 (CASP6) Bos taurus; Bovine (Cattle)
KL353Ra02 Slit同源物3(Slit3)重組蛋白 Recombinant Slit Homolog 3 (Slit3) Rattus norvegicus (Rat)
KL354Hu02 晶狀體蛋白λ1(CRYλ1)重組蛋白 Recombinant Crystallin Lambda 1 (CRYl1) Homo sapiens (Human)
KL008Mu02 血管生成素1(ANGPT1)重組蛋白 Recombinant Angiopoietin 1 (ANGPT1) Mus musculus (Mouse)
KL021Hu02 RNA結(jié)合基元蛋白20(RBM20)重組蛋白 Recombinant RNA Binding Motif Protein 20 (RBM20) Homo sapiens (Human)
KL150Hu02 癌胚抗原(CEA)重組蛋白 Recombinant Carcinoembryonic Antigen (CEA) Homo sapiens (Human)
KL557Mu03 甲狀腺過氧化物酶(TPO)重組蛋白 Recombinant Thyroid Peroxidase (TPO) Mus musculus (Mouse)
KL988Hu02 細(xì)胞色素P450家族成員2E1(CYP2E1)重組蛋白 Recombinant Cytochrome P450 2E1 (CYP2E1) Homo sapiens (Human)
KL785Hu02 腦啡肽酶(NEP)重組蛋白 Recombinant Neprilysin (NEP) Homo sapiens (Human)
KL889Hu02 周期素依賴性激酶2(CDK2)重組蛋白 Recombinant Cyclin Dependent Kinase 2 (CDK2) Homo sapiens (Human)
KL475Hu02 甘氨酸脫氫酶(GLDC)重組蛋白 Recombinant Glycine Dehydrogenase (GLDC) Homo sapiens (Human)
KL716Hu02 亮氨酸豐富重復(fù)激酶2(LRRK2)重組蛋白 Recombinant Leucine Rich Repeat Kinase 2 (LRRK2) Homo sapiens (Human)
