大鼠胰島素樣生長因子1(IGF-1)化學(xué)發(fā)光**分析試劑盒
基本原理:
本試劑盒采用雙抗體夾心法。用抗大鼠IGF-1抗體包被于酶標(biāo)板上,實(shí)驗(yàn)時標(biāo)本或標(biāo)準(zhǔn)品中的IGF-1會與包被抗體結(jié)合,游離的成分被洗去。依次加入生物素化的抗大鼠IGF-1抗體和辣根過氧化物酶標(biāo)記的親和素。抗大鼠IGF-1抗體與結(jié)合在包被抗體上的大鼠IGF-1結(jié)合、生物素與親和素特異性結(jié)合而形成**復(fù)合物,游離的成分被洗去。加入發(fā)光底物混合液,發(fā)光底物在辣根過氧化物酶的催化下發(fā)出熒光,用化學(xué)發(fā)光**分析儀測定化學(xué)發(fā)光值(RLU),IGF-1濃度與化學(xué)發(fā)光值之間呈正相關(guān),通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中IGF-1的濃度。
大鼠胰島素樣生長因子1(IGF-1)化學(xué)發(fā)光**分析試劑盒描述:
英文名稱 | Rat IGF-1 (Insulin-Like Growth Factor 1) CLIA Kit | ||
中文名稱 | 大鼠胰島素樣生長因子1(IGF-1)化學(xué)發(fā)光**分析試劑盒 | ||
貨號 | KL-R0010c | 種屬 | Rat/大鼠 |
規(guī)格 | 96T/Kit (8*12 wells) | ||
檢測方法 | 雙抗體夾心法 | ||
檢測范圍 | 12.5~800pg/mL | 靈敏度 | 7.5pg/mL |
大鼠胰島素樣生長因子1(IGF-1)化學(xué)發(fā)光**分析試劑盒試劑盒組成:
中文名稱 | 英文名稱 | 規(guī)格 | 保存 |
CLIA酶標(biāo)板 | Micro CLIA Plate | 8×12 wells | 4℃/-20℃ # |
凍干標(biāo)準(zhǔn)品 | Reference Standard | 2 支 | 4℃/-20℃ # |
標(biāo)準(zhǔn)品&樣品稀釋液 | Reference Standard & Sample Diluent | 1瓶 20mL | 4℃ |
濃縮生物素化抗體 | Concentrated Biotinylated Detection Ab | 1支 120μL | 4℃/-20℃ # |
生物素化抗體稀釋液 | Biotinytated Detection Ab Diluent | 1瓶10mL | 4℃ |
濃縮HRP酶結(jié)合物 | Concentrated HRP Conjugate | 1支 120μL | 4℃(避光) |
酶結(jié)合物稀釋液 | HRP Conjugate Diluent | 1瓶 10mL | 4℃ |
濃縮洗滌液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL | 4℃ |
發(fā)光底物A液 | Substrate Reagent A | 1瓶 5mL | 4℃(避光) |
發(fā)光底物B液 | Substrate Reagent B | 1瓶 5mL | 4℃(避光) |
封板覆膜 | Plate Sealer | 5 張 |
|
產(chǎn)品說明書 | Manual | 1 份 |
|
質(zhì)檢報告 | Certificate of Analysis | 1 份 |
|
特別說明: #: 一周內(nèi)使用可存于4℃,需長時間存放或多次使用建議存于-20℃. |
1.在各孔中加入標(biāo)準(zhǔn)品或樣品各 100μL,37℃孵育90分鐘
2. 倒去孔內(nèi)液體,拍干,加入 100μL 生物素化抗體工作液,37℃孵育 60 分鐘
3. 洗滌 3 次
4. 加入 100μL 酶結(jié)合物工作液, 37℃孵育 30 分鐘
5. 洗滌 5 次
6. 加入 100μL 發(fā)光底物混合液, 37℃孵育 5 分鐘左右
7. 立即測定各孔的化學(xué)發(fā)光值
8. 結(jié)果計算
KL-a2PI-b 牛α2-纖溶酶抑制因子(α2PI) 檢測試劑盒 Bovine Alpha-2-Plasmin Inhibitor (a2PI) ELISA Kit Bos taurus; Bovine Cattle 31.25-2000 ng/mL 11.32 ng/mL 96T 12 months
KL-PROCR-Mu 小鼠血管內(nèi)皮細(xì)胞蛋白C受體(PROCR) 檢測試劑盒 mouse Protein C Receptor, Endothelial (PROCR) ELISA Kit Mus musculus mouse 78.125-5000pg/mL 28.6pg/mL 96T 12 months
KL-EG-VEGF-Hu 人***腺來源血管內(nèi)皮生長因子(EG-VEGF) 檢測試劑盒 human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit Homo sapiens human 15.625-1000pg/mL 7.2pg/mL 96T 12 months
KL-ECF-Hu 人嗜酸粒細(xì)胞趨化因子(ECF) 檢測試劑盒 human Eosinophil Chemotactic Factor (ECF) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.2pg/mL 96T 12 months
KL-ECF-Mu 小鼠嗜酸粒細(xì)胞趨化因子(ECF) 檢測試劑盒 mouse Eosinophil Chemotactic Factor (ECF) ELISA Kit Mus musculus mouse 31.25-2000pg/mL 13.9pg/mL 96T 12 months
KL-CCL3L1-Hu 人趨化因子C-C-基元配體3樣蛋白1(CCL3L1) 檢測試劑盒 human Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit Homo sapiens human 78.125-5000pg/mL 32pg/mL 96T 12 months
KL-CCL3L1-Mu 小鼠趨化因子C-C-基元配體3樣蛋白1(CCL3L1) 檢測試劑盒 mouse Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit Mus musculus mouse 78.125-5000pg/mL 35pg/mL 96T 12 months
KL-EPOR-Hu 人紅細(xì)胞生成素受體(EPOR) 檢測試劑盒 human Erythropoietin Receptor (EPOR) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.057ng/mL 96T 12 months
KL-EPO-Hu 人紅細(xì)胞生成素(EPO) 檢測試劑盒 human Erythropoietin (EPO) ELISA Kit Homo sapiens human 6.25-400pg/mL 2.36pg/mL 96T 12 months
KL-EPO-Mu 小鼠紅細(xì)胞生成素(EPO) 檢測試劑盒 mouse Erythropoietin (EPO) ELISA Kit Mus musculus mouse 6.25-400pg/mL 2.65pg/mL 96T 12 months
KL-SELE-Hu 人E選擇素(SELE) 檢測試劑盒 human Selectin, Endothelium (SELE) ELISA Kit Homo sapiens human 39.063-2500pg/mL 14pg/mL 96T 12 months
KL-SELE-Mu 小鼠E選擇素(SELE) 檢測試劑盒 mouse Selectin, Endothelium (SELE) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.051ng/mL 96T 12 months
KL-FAS-Hu 人凋亡相關(guān)因子(FAS) 檢測試劑盒 human Factor Related Apoptosis (FAS) ELISA Kit Homo sapiens human 39.063-2500pg/mL 12pg/mL 96T 12 months
KL-FAS-Mu 小鼠凋亡相關(guān)因子(FAS) 檢測試劑盒 mouse Factor Related Apoptosis (FAS) ELISA Kit Mus musculus mouse 31.25-2000pg/mL 10.2pg/mL 96T 12 months
KL-FASL-Hu 人凋亡相關(guān)因子配體(FASL) 檢測試劑盒 human Factor Related Apoptosis Ligand (FASL) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.8pg/mL 96T 12 months
KL-FASL-Mu 小鼠凋亡相關(guān)因子配體(FASL) 檢測試劑盒 mouse Factor Related Apoptosis Ligand (FASL) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.060ng/mL 96T 12 months
KL-FGF1-Hu 人酸性成纖維細(xì)胞生長因子(FGF1) 檢測試劑盒 human Fibroblast Growth Factor 1, Acidic (FGF1) ELISA Kit Homo sapiens human 15.625-1000pg/mL 6.1pg/mL 96T 12 months
KL-FGF4-Hu 人成纖維細(xì)胞生長因子4(FGF4) 檢測試劑盒 human Fibroblast Growth Factor 4 (FGF4) ELISA Kit Homo sapiens human 31.25-2000pg/mL 14.5pg/mL 96T 12 months
KL-FGF6-Hu 人成纖維細(xì)胞生長因子6(FGF6) 檢測試劑盒 human Fibroblast Growth Factor 6 (FGF6) ELISA Kit Homo sapiens human 7.813-500pg/mL 3.3pg/mL 96T 12 months
KL-FGF9-Hu 人成纖維細(xì)胞生長因子9(FGF9) 檢測試劑盒 human Fibroblast Growth Factor 9 (FGF9) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.064ng/mL 96T 12 months
KL-FN-Hu 人纖連蛋白(FN) 檢測試劑盒 human Fibronectin (FN) ELISA Kit Homo sapiens human 1.563-100ng/mL 0.72ng/mL 96T 12 months
KL-FN-Mu 小鼠纖連蛋白(FN) 檢測試劑盒 mouse Fibronectin (FN) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.062ng/mL 96T 12 months
KL-FN-Ra 大鼠纖連蛋白(FN) 檢測試劑盒 Rat Fibronectin (FN) ELISA Kit Rattus norvegicus Rat 12.5-800ng/mL 5.61ng/mL 96T 12 months
KL-Flt3L-Hu 人FMS樣酪氨酸激酶3配體(Flt3L) 檢測試劑盒 human FMS Like Tyrosine Kinase 3 Ligand (Flt3L) ELISA Kit Homo sapiens human 15.625-1000pg/mL 7.5pg/mL 96T 12 months