產(chǎn)品詳情
簡單介紹：
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗體產(chǎn)品質(zhì)量穩(wěn)定，實驗效果明顯，貨期快，價格優(yōu)惠，歡迎垂詢訂購！我公司長期供應**組化抗體、WB抗體、**組化試劑盒和抗體試驗所需全部相關試劑、熒光標記抗體、單克隆抗體、多克隆抗體、各種標記的二抗IgG/IgM/IgD/IgA等科研實驗抗體。Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗體用于**組化實驗,WB實驗,相應的標記抗體有HRP標記抗體,FITC標記,BIO等。
詳情介紹：
Rabbit Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)
Cat. Number:
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗體KL-6582R
Quantity size:
0.1ml
Concentration:
1mg/ml Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗體element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Also known as:
p-Rpb1 CTD (Ser2/Ser5); DNA directed RNA polymerase II A; p-Rpb1 CTD(Ser2/Ser5); DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗體DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN.
Specificity:
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Rabbit Polyclonal IgG, affinity purified by Protein A.
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Reacts with: Human, Mouse, Rat, Dog, Cow, Rabbit, .
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Immunogen: KLH conjugated Synthesised Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗體phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5..
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Predicted Molecular Weight: 240kDa.
Storage:
Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).
Application:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
Not yet tested in other applications. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗體
Optimal working dilutions must be determined by the end user.
Not yet tested in other applications. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗體
Optimal working dilutions must be determined by the end user.
