產(chǎn)品詳情
簡(jiǎn)單介紹:
重組人甲狀旁腺與***相關(guān)的蛋白質(zhì)蛋白與其它公司提供的重組蛋白不同,rHuPTHrP蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白,可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效,將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組人甲狀旁腺與***相關(guān)的蛋白質(zhì)蛋白(全長(zhǎng)序列)產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹:
重組人甲狀旁腺與***相關(guān)的蛋白質(zhì)蛋白
Synonyms | Parathormone, Parathyrin |
Species | Human |
Accession | P12272 |
GeneID | 5744 |
Source | Escherichia coli. |
Molecular Weight | 重組人甲狀旁腺與***相關(guān)的蛋白質(zhì)蛋白Approximately 9.9 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. |
Quantity | 10μg/50μg/1000μg |
AA Sequence | AVSEHQLLHD KGKSIQDLRR RFFLHHLIAE IHTAEIRATS EVSPNSKPSP NTKNHPVRFG SDDEGRYLTQ ETNKVETYKE QPLKTP |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity | Data not available. |
Physical Appearance | 重組人甲狀旁腺與***相關(guān)的蛋白質(zhì)蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 6.0, 300 mM NaCl. |
Endotoxin | Less than 0.1 EU/μg of rHuPTHrP as determined by LAL method. |
Reconstitution | 重組人甲狀旁腺與***相關(guān)的蛋白質(zhì)蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
重組人甲狀旁腺與***相關(guān)的蛋白質(zhì)蛋白 | |
SDS-PAGE |