產(chǎn)品詳情
簡單介紹：
重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白與其它公司提供的重組蛋白不同，rHuENA-78, 8-78 a.a./CXCL5蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白
| Synonyms | CXCL5, Small-inducible cytokine B5 |
| Species | Human |
| Accession | P42830 |
| GeneID | 6374 |
| Source | 重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白Escherichia coli. |
| Molecular Weight | Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10.0-100.0 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuENA-78, 8 - 78 a.a./CXCL5 as determined by LAL method. |
| Reconstitution | 重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人上皮中性粒細胞激活肽-78,8-78a.a./CXCL5蛋白 | |
| Reference |
1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82. 2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 3. Persson T, Monsef N, Andersson P, et al. 2003. Clin Exp Allergy. 33:531-7. 4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9. |
| Background | CXCL5 is a member of the CXC chemokine family and also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. In vitro, ENA-78 (8-78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. They are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Recombinant human CXCL5 (8-78 a.a.) contains 71 amino acids which is a single non-glycosylated polypeptide chain. Human CXCL5 shares 57 % amino acid sequence identity with mouse and rat CXCL5. |
