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產品名稱：重組人類B細胞成熟抗原/ TNFRSF17蛋白
產品型號：rHuBCMA/TNFRSF17
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人類B細胞成熟抗原/ TNFRSF17蛋白與其它公司提供的重組蛋白不同，rHuBCMA/TNFRSF17蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類B細胞成熟抗原/ TNFRSF17蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人類B細胞成熟抗原/ TNFRSF17蛋白

Synonyms TNFRSF17, BCM, CD269

Species Human

Accession Q02223

GeneID 608

Source Escherichia coli.

Molecular Weight 重組人類B細胞成熟抗原/ TNFRSF17蛋白Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人類B細胞成熟抗原/ TNFRSF17蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 10⁴ IU/mg in the presence of 100 ng/ml human APRIL.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.

Endotoxin 重組人類B細胞成熟抗原/ TNFRSF17蛋白Less than 1 EU/μg of rHuBCMA as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人類B細胞成熟抗原/ TNFRSF17蛋白

Reference 1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44: 69-81.
2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346: 1158-62.
3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278: 33127-33.
4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22: 1147-54.

Background B-Cell Maturation Antigen (BCMA), encoded by the TNFRSF17 gene in humans. It is a member of the TNF receptor superfamily and a type III membrane protein containing one extracellular cysteine rich domain. It is expressed on mature B-cells and other B-cell lines. BCMA can binds to TNFSF13B/BLyS/BAFF and TNFSF13/APRIL, and has functions that promotes B-cell survival, plays a role in the regulation of humoral immunity, and activates NF-kappa-B and JNK BCMA.

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Synonyms	TNFRSF17, BCM, CD269
Species	Human
Accession	Q02223
GeneID	608
Source	Escherichia coli.
Molecular Weight	重組人類B細胞成熟抗原/ TNFRSF17蛋白Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人類B細胞成熟抗原/ TNFRSF17蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 10⁴ IU/mg in the presence of 100 ng/ml human APRIL.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
Endotoxin	重組人類B細胞成熟抗原/ TNFRSF17蛋白Less than 1 EU/μg of rHuBCMA as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類B細胞成熟抗原/ TNFRSF17蛋白
Reference	1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44: 69-81. 2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346: 1158-62. 3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278: 33127-33. 4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22: 1147-54.
Background	B-Cell Maturation Antigen (BCMA), encoded by the TNFRSF17 gene in humans. It is a member of the TNF receptor superfamily and a type III membrane protein containing one extracellular cysteine rich domain. It is expressed on mature B-cells and other B-cell lines. BCMA can binds to TNFSF13B/BLyS/BAFF and TNFSF13/APRIL, and has functions that promotes B-cell survival, plays a role in the regulation of humoral immunity, and activates NF-kappa-B and JNK BCMA.

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產品名稱：重組人類B細胞成熟抗原/ TNFRSF17蛋白