產(chǎn)品詳情
簡(jiǎn)單介紹：
重組小鼠生長(zhǎng)分化因子5 /骨形態(tài)發(fā)生蛋白-14蛋白與其它公司提供的重組蛋白不同，rMuGDF-5/BMP-14蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組小鼠生長(zhǎng)分化因子5 /骨形態(tài)發(fā)生蛋白-14蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組小鼠生長(zhǎng)分化因子5 /骨形態(tài)發(fā)生蛋白-14蛋白
| Species | Murine |
| Accession | P43027 |
| GeneID | 14563 |
| Source | 重組小鼠生長(zhǎng)分化因子5 /骨形態(tài)發(fā)生蛋白-14蛋白Escherichia coli. |
| Molecular Weight | Approximately 27.2 kDa, a disulfide-linked homodimeric protein containing two 120 amino acids. |
| AA Sequence | APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR |
| Purity | 重組小鼠生長(zhǎng)分化因子5 /骨形態(tài)發(fā)生蛋白-14蛋白> 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA. |
| Endotoxin | Less than 0.1 EU/μg of rMuGDF-5/BMP-14 as determined by LAL method. |
| Reconstitution | 重組小鼠生長(zhǎng)分化因子5 /骨形態(tài)發(fā)生蛋白-14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組小鼠生長(zhǎng)分化因子5 /骨形態(tài)發(fā)生蛋白-14蛋白 | |
| Background | Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems. |
