產(chǎn)品詳情
簡(jiǎn)單介紹：
重組鼠巨噬細(xì)胞炎癥蛋白-1 gamma / CCL9 / CCL10蛋白與其它公司提供的重組蛋白不同，rMuMIP-1γ/CCL9/CCL10蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組鼠巨噬細(xì)胞炎癥蛋白-1 gamma / CCL9 / CCL10蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組鼠巨噬細(xì)胞炎癥蛋白-1 gamma / CCL9 / CCL10蛋白
| Synonyms | MIP-1-gamma, MRP-2, Small-inducible Cytokine A9 |
| Species | Murine |
| Accession | P51670 |
| GeneID | 20308 |
| Source | 重組鼠巨噬細(xì)胞炎癥蛋白-1 gamma / CCL9 / CCL10蛋白Escherichia coli. |
| Molecular Weight | Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠巨噬細(xì)胞炎癥蛋白-1 gamma / CCL9 / CCL10蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method. |
| Reconstitution | 重組鼠巨噬細(xì)胞炎癥蛋白-1 gamma / CCL9 / CCL10蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠巨噬細(xì)胞炎癥蛋白-1 gamma / CCL9 / CCL10蛋白 | |
| SDS-PAGE |
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| Reference |
1. Youn BS, Jang IK, Broxmeyer HE, et al. 1995. J Immunol. 155:2661-7. 2. Berahovich RD, Miao Z, Wang Y, et al. 2005. J Immunol. 174:7341-51. 3. Lean JM, Murphy C, Fuller K, et al. 2002. J Cell Biochem. 87:386-93. |
| Background | Murine CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine CCL9/10 is 75 % amino acid identical to rat CCL9/10. |
