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產(chǎn)品詳情
  • 產(chǎn)品名稱:重組鼠單核細(xì)胞趨化蛋白-5 / CCL12蛋白

  • 產(chǎn)品型號(hào):rMuMCP-5/CCL12
  • 產(chǎn)品廠商:KALANG
  • 產(chǎn)品文檔:
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簡單介紹:
重組鼠單核細(xì)胞趨化蛋白-5 / CCL12蛋白與其它公司提供的重組蛋白不同,rMuMCP-5/CCL12蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白,可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡便高效,將蛋白純化過程中對(duì)蛋白的損傷降低到*小程度.重組鼠單核細(xì)胞趨化蛋白-5 / CCL12蛋白(全長序列)產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測、ELISA等試驗(yàn)中.
詳情介紹:

重組鼠單核細(xì)胞趨化蛋白-5 / CCL12蛋白

Synonyms MCP-1-related Chemokine, Small-inducible Cytokine A12
Species Murine
Accession Q62401
GeneID 20293
Source 重組鼠單核細(xì)胞趨化蛋白-5 / CCL12蛋白Escherichia coli.
Molecular Weight Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組鼠單核細(xì)胞趨化蛋白-5 / CCL12蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method.
Reconstitution 重組鼠單核細(xì)胞趨化蛋白-5 / CCL12蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠單核細(xì)胞趨化蛋白-5 / CCL12蛋白
Reference 1. Sarafi MN, Garcia-Zepeda EA, MacLean JA, et al. 1997. J Exp Med. 185:99-109.
2. Jia GQ, Gonzalo JA, Lloyd C, et al. 1996. J Exp Med. 184:1939-51.
Background Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2.

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