產品詳情
簡單介紹：
重組大鼠胸腺和激活調節(jié)的趨化因子/ CCL17蛋白與其它公司提供的重組蛋白不同，rRtTARC/CCL17蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠胸腺和激活調節(jié)的趨化因子/ CCL17蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠胸腺和激活調節(jié)的趨化因子/ CCL17蛋白
| Species | Rat |
| Accession | Q9ERE0 |
| GeneID | 117518 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠胸腺和激活調節(jié)的趨化因子/ CCL17蛋白Approximately 8.1 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | ARATNVGREC CLDYFKGAIP IRKLVTWFRT SVECPKDAIV FETVQGRLIC TDPKDKHVKK AIRHLKNQRL |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠胸腺和激活調節(jié)的趨化因子/ CCL17蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | 重組大鼠胸腺和激活調節(jié)的趨化因子/ CCL17蛋白Less than 0.1 EU/μg of r rRtTARC/CCL17 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠胸腺和激活調節(jié)的趨化因子/ CCL17蛋白 | |
| SDS-PAGE |
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| Reference |
1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308. 2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21. 3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21. 4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8. 5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8. |
| Background | CCL17 also known as thymus and activation-related chemokine (TARC) is encoded by the CCL17 gene located on the chromosome 16. It is expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. CCL17 signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. It plays an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides. CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309. |
