產(chǎn)品詳情
簡單介紹：
重組人類生長調(diào)節(jié)蛋白-γ/ CXCL3蛋白與其它公司提供的重組蛋白不同，rHuGRO-γ/CXCL3蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨(dú)有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類生長調(diào)節(jié)蛋白-γ/ CXCL3蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類生長調(diào)節(jié)蛋白-γ/ CXCL3蛋白
| Synonyms | GRO-gamma, MIP2-beta |
| Species | Human |
| Accession | P19876 |
| GeneID | 2921 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人類生長調(diào)節(jié)蛋白-γ/ CXCL3蛋白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人類生長調(diào)節(jié)蛋白-γ/ CXCL3蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuGRO-γ/CXCL3 as determined by LAL method. |
| Reconstitution | 重組人類生長調(diào)節(jié)蛋白-γ/ CXCL3蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類生長調(diào)節(jié)蛋白-γ/ CXCL3蛋白 | |
| SDS-PAGE |
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| Reference |
1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6. 2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83. 3. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84. 4. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9. |
| Background | CXCL3, also named GRO-γ, is belonging to the CXC chemokine family and encoded by the gene CXCL3. CXCL3/GRO-γ shares 86 % amino acid sequence with CXCL1/GRO-α. All three human GROs (GRO-α, GRO-β, GRO-γ) are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is secreted by monocytes and macrophages. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. CXCL3 plays a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three GROs can bind with high affinity to the IL-8 receptor type B. |
