產(chǎn)品詳情
簡單介紹：
重組人腦鈉素肽蛋白與其它公司提供的重組蛋白不同，rHuBNP蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人腦鈉素肽蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人腦鈉素肽蛋白
| Synonyms | Brain natriuretic peptide 32, Gamma-brain natriuretic peptide, B-type Natriuretic Peptide, GC-B, BNP-32 |
| Species | Human |
| Accession | P16860 |
| GeneID | 4879 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人腦鈉素肽蛋白Approximately 3.5 kDa, a single non-glycosylated polypeptide chain containing 32 amino acids. |
| Quantity | 20μg/100μg/1000μg |
| AA Sequence | SPKMVQGSGC FGRKMDRISS SSGLGCKVLR RH |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人腦鈉素肽蛋白Data Not Available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuBNP as determined by LAL method. |
| Reconstitution | 重組人腦鈉素肽蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人腦鈉素肽蛋白 | |
| Reference |
1. Matsuo H, Kangawa K, Minamino N. 1988. Tanpakushitsu Kakusan Koso, 33: 2438-50. 2. Uehara Y, Shimizu H, Shimomura Y, et al. 1990. Neuropeptides, 17: 107-10. 3. Hunt PJ, Espiner EA, Nicholls MG, et al. 1997. Peptides, 18: 1475-81. 4. Richards AM, Lainchbury JG, Nicholls MG, et al. 2002. Trends Endocrinol Metab, 13: 151-5. 5. Trojnarska O, Gwizdala A, Katarzynski S, et al. 2010. Int J Cardiol, 139: 241-7. |
| Background | Brain Natriuretic Peptide is encoded by the BNP gene located on the Chr.1 in humans. It is firstly discovered in the porcine brain and given this name, but the protein is mainly expressed in the cardiac ventricles in human body after the excessive stretching of cardiomyocytes. The gene expresses a 134 a.a. sequence which contains a 1-26 a.a. signal peptide and 27-134 a.a. Natriuretic peptides B, and the BNP is the 32 a.a. C-terminus of natriuretic peptides B. The BNP can be cleaved in 16 chains and the rHuBNP is 1-32. BNP acts as a cardiac hormone with a variety of functions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Additionnaly, it plays a key role in cardiovascular homeostasis, helps restore the body's salt and water balance and improves heart function. |
