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產(chǎn)品名稱：重組人白細(xì)胞介素-11蛋白
產(chǎn)品型號：rHuIL-11
產(chǎn)品廠商：KALANG
產(chǎn)品文檔：

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簡單介紹：

重組人白細(xì)胞介素-11蛋白與其它公司提供的重組蛋白不同，rHuIL-11蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人白細(xì)胞介素-11蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人白細(xì)胞介素-11蛋白

Synonyms AGIF

Species Human

Accession P20809

GeneID 3589

Source Escherichia coli.

Molecular Weight 重組人白細(xì)胞介素-11蛋白Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人白細(xì)胞介素-11蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine B9-11 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rHuIL-11 as determined by LAL method.

Reconstitution 重組人白細(xì)胞介素-11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人白細(xì)胞介素-11蛋白

Reference 1. McKinley D, Wu Q, Yang-Feng T, et al. 1992. Genomics. 13:814-9.
2. Paul SR, Bennett F, Calvetti JA, et al. 1990. Proc Natl Acad Sci U S A. 87:7512-6.
3. Barton VA, Hall MA, Hudson KR, et al. 2000. J Biol Chem. 275:36197-203.

Background Interleukin-11 (IL-11) is encoded by the IL11 gene and is a member of IL-6 superfamily, distinguished based on their use of the common co-receptor gp130. It is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11Rα subunit and two molecules of the expressed cell signaling β subunit, gp130. The functions of IL-11 are that it directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production.

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Synonyms	AGIF
Species	Human
Accession	P20809
GeneID	3589
Source	Escherichia coli.
Molecular Weight	重組人白細(xì)胞介素-11蛋白Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人白細(xì)胞介素-11蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine B9-11 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rHuIL-11 as determined by LAL method.
Reconstitution	重組人白細(xì)胞介素-11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人白細(xì)胞介素-11蛋白
Reference	1. McKinley D, Wu Q, Yang-Feng T, et al. 1992. Genomics. 13:814-9. 2. Paul SR, Bennett F, Calvetti JA, et al. 1990. Proc Natl Acad Sci U S A. 87:7512-6. 3. Barton VA, Hall MA, Hudson KR, et al. 2000. J Biol Chem. 275:36197-203.
Background	Interleukin-11 (IL-11) is encoded by the IL11 gene and is a member of IL-6 superfamily, distinguished based on their use of the common co-receptor gp130. It is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11Rα subunit and two molecules of the expressed cell signaling β subunit, gp130. The functions of IL-11 are that it directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production.

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產(chǎn)品名稱：重組人白細(xì)胞介素-11蛋白