產(chǎn)品詳情
簡單介紹：
重組人泛素偶聯(lián)酶E2 B，His蛋白與其它公司提供的重組蛋白不同，rHuUBE2B, His蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人泛素偶聯(lián)酶E2 B，His蛋白（全長序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測、ELISA等試驗(yàn)中.
詳情介紹：
重組人泛素偶聯(lián)酶E2 B，His蛋白
| Synonyms | HR6B, Ubiquitin Carrier Protein B, Ubiquitin-protein Ligase B |
| Species | Human |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 19.0 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids of human UBE2B and 14 a.a. vector sequence including 6 × His tag at N-terminus. |
| Quantity | 10μg/50μg/1000μg |
| AA Sequence | 重組人泛素偶聯(lián)酶E2 B，His蛋白MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS |
| Concentration | See label. |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data is not available. |
| Physical Appearance | Sterile Colorless liquid. |
| Formulation | 重組人泛素偶聯(lián)酶E2 B，His蛋白A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 7.6 with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT. |
| Endotoxin | Less than 1 EU/μg of rHuUBE2B, His as determined by LAL method. |
| Storage | Store at -20 °C. |
| 重組人泛素偶聯(lián)酶E2 B，His蛋白 | |
| SDS-PAGE |
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| Reference |
重組人泛素偶聯(lián)酶E2 B，His蛋白1. Roest HP, van Klaveren J, de Wit J, et al. 1996. Cell, 86: 799-810. 2. Adegoke OA, Bedard N, Roest HP, et al. 2002. Am J Physiol Endocrinol Metab, 283: E482-9. 3. Kavakebi P, Hausott B, Tomasino A, et al. 2005. Mol Cell Neurosci, 29: 559-68. 4. Mulugeta Achame E, Wassenaar E, Hoogerbrugge JW, et al. 2010. BMC Genomics, 11: 367. |
| Background | Ubiquitin-conjugating enzyme E2 B belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2B gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2B interacts with RAD18, UBR2 and WAC. Its protein sequence is 100 % identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. |
