產(chǎn)品詳情
簡單介紹：
重組鼠角質細胞生長因子-2 / FGF-10蛋白與其它公司提供的重組蛋白不同，rMuKGF-2/FGF-10蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠角質細胞生長因子-2 / FGF-10蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠角質細胞生長因子-2 / FGF-10蛋白
| Species | Murine |
| Accession | O35565 |
| GeneID | 14165 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately19.5 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids. |
| Quantity | 重組鼠角質細胞生長因子-2 / FGF-10蛋白5μg/25μg/1000μg |
| AA Sequence | QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠角質細胞生長因子-2 / FGF-10蛋白Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol. |
| Endotoxin | Less than 1 EU/μg of rMuKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution | 重組鼠角質細胞生長因子-2 / FGF-10蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠角質細胞生長因子-2 / FGF-10蛋白 | |
| Reference |
1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4. 2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404. 3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63. 4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5. |
| Background | Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family, which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may take parts in wound healing and is required for normal branching morphogenesis. Recombinant murine FGF-10 contains a 173 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and rat FGF-10. |
