產品詳情
簡單介紹：
重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白與其它公司提供的重組蛋白不同，rRtEotaxin/CCL11蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白
| Synonyms | Small-inducible Cytokine A11 |
| Species | Rat |
| Accession | P97545 |
| GeneID | 29397 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | 重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Less than 1 EU/μg of rRtEotaxin/CCL11 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠嗜酸性粒細胞趨化因子/ CCL11蛋白 | |
| SDS-PAGE |
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| Reference |
1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12. 2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7. 3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56. 4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5. |
| Background | CCL11 encoded by the gene CCL11is belonging to the CC chemokine family. It is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. CCL11 is a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. CCR3 has been identified to be a specific CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates. |
